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Electrotransfer to nitrocellulose membrane Volumes are for 10 cm x 10 cm cm2 of membrane; for different sized membranes, adjust volumes accordingly. Incubate membrane in 25 ml of blocking buffer for 1 hr at room temperature. Wash Site de rencontre emirats arabes unis times emifats 5 min each with 15 ml of TBST. Proceed with detection Section D. Detection of Proteins Directions umis Use: Incubate substrate with membrane for 1 minute, remove excess solution membrane remains wetwrap in plastic and expose to X-ray film.
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